Coding
ecf16

Part:BBa_K2070001:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)


ecf16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part sequence was designed with reference to the amnio acid sequence of ECF sigma provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.

Source

Pseudomonas entomophila L48

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58